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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 321112).
Application References:
1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB IHC Block) 2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
GM-CSF-stimulated (day-3) human monocytes stained with 15-2 Alexa Fluor® 488
Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD.
Distribution:
Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function:
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand Receptor:
Mannose, fucose, N-acetyl glucosamine
Antigen References:
1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303 2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469. 4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. 5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD206 (MMR)
GM-CSF-stimulated (day-3) human monocytes stained with purified 15-2, followed by anti-mouse IgG FITC
FITC anti-human CD206 (MMR)
GM-CSF stimulated (day3) human peripheral blood monocytes stained with 15-2 FITC
PE anti-human CD206 (MMR)
GM-CSF-stimulated (day-3) human monocytes stained with 15-2 PE
PE/Cy5 anti-human CD206 (MMR)
GM-CSF-stimulated human peripheral blood monocytes (day-3) stained with 15-2 PE/Cy5
APC anti-human CD206 (MMR)
GM-CSF- stimulated (day-3) human monocytes stained with 15-2 APC
Alexa Fluor® 488 anti-human CD206 (MMR)
GM-CSF-stimulated (day-3) human monocytes stained with 15-2 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD206 (MMR)
GM-CSF-stimulated (day-3) human monocytes stained with 15-2 Alexa Fluor® 647
Biotin anti-human CD206 (MMR)
GM-CSF-stimulated human peripheral blood monocytes (day-3) stained with biotinylated 15-2, followed by Sav-PE
APC/Cy7 anti-human CD206 (MMR)
GM-CSF- stimulated (day-3) human monocytes stained with 15-2 APC/Cy7
PerCP/Cy5.5 anti-human CD206 (MMR)
GM-CSF-stimulated (3 days) human peripheral blood monocytes were stained with CD206 (clone 15-2) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).
PE/Cy7 anti-human CD206 (MMR)
GM-CSF-stimulated (3 days) human peripheral blood monocytes were stained with CD206 (clone 15-2) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
