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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 306310).
Application References:
1. Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim) 2. Vincent S, et al. 2002. J. Virol. 76:6121. 3. Cocks B, et al. 1995. Nature 376:260. 4. Sayos J, et al. 2001. Blood 97:3867. (IP) 5. Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim) 6. Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed
Human peripheral blood lymphocytes stained with A12 Alexa Fluor® 488
CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with biotinylated A12, followed by Sav-PE
FITC anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 FITC
LEAF™ Purified anti-human CD150 (SLAM)
Human peripheral blood lymphocytes LEAF™ purified A12, followed by biotinylated anti-mouse IgG and Sav-PE
PE anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 PE
Purified anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with purified A12, followed by biotinylated anti-mouse IgG and Sav-PE
Alexa Fluor® 488 anti-human CD150 (SLAM)
Human peripheral blood lymphocytes stained with A12 Alexa Fluor® 488
