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Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM) Antibody
Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM) Antibody
342007 25 tests $110.00 Qty:
    
342008 100 tests $235.00 Qty:
    

Product Details

 
Clone: SHM-57 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:Alexa Fluor® 488 Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM) Antibody SHM-57 Image 1
Human cervical cancer cell line, Hela, stained with SHM-57 Alexa Fluor® 488


Compare all formats

See Alexa Fluor® 488 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD146 is a 118 kD integral transmembrane glycoprotein also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Other Names: Muc-18, MCAM, Mel-CAM, S-endo
Structure: 118 kD integral glycoprotein, Ig superfamily
Distribution: Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells
Function: Adhesion, monocytes transendothelial migration
Antigen
References:

1. Pickl WF, et al. 1997. J. Immunol. 158:2107
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746

GeneID: 4162
Latest Publications: View the latest CD146 articles on HighwirePress.com
UniProt: View information about CD146 on UniProt.org
Keywords: Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM), SHM-57, Alexa Fluor® 488, AF488, Muc-18, MCAM, Mel-CAM, S-endo, Human, Flow Cytometry, Immunology, Antibodies


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Related Products

DescriptionCloneApplications
Alexa Fluor® 488 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 647 anti-human CD146 (MUC18, Mel-CAM)SHM-57FC
APC anti-human CD146 (MUC18, Mel-CAM)SHM-57FC
PE anti-human CD146 (MUC18, Mel-CAM)SHM-57FC
PE/Cy7 anti-human CD146 (MUC18, Mel-CAM)SHM-57FC
PerCP/Cy5.5 anti-human CD146 (MUC18, Mel-CAM)SHM-57FC
Purified anti-human CD146 (MUC18, Mel-CAM)SHM-57FC


Related Pathways

Pathway
Stem Cells and Development
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > CD and Related Molecules > CD146 > SHM-57
Immunology > Human Immunology > Adhesion Molecules > CD146 > SHM-57

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with purified SHM-57 conjugated with PE

  • PE anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with SHM-57 PE

  • Alexa Fluor® 647 anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with SHM-57 Alexa Fluor® 647

  • Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with SHM-57 Alexa Fluor® 488

  • PE/Cy7 anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with SHM-57 PE/Cy7

  • APC anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, Hela, stained with CD146 (clone SHM-57) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

  • PerCP/Cy5.5 anti-human CD146 (MUC18, Mel-CAM)
    Human cervical cancer cell line,

    Human cervical cancer cell line, HeLa, stained with purified CD146 (clone SHM-57) PerCP/Cy5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).

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