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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
Hela cells were treated with 20 µM of Nocodazole for 24 hours then fixed, permeabilized with 70% ethanol, and then stained with anti-Histone H3 Phospho-Ser10 (clone 11D8) Alexa Flour® 488 or mouse IgG2b, κ Alexa Flour® 488 isotype control.
Histone H3 is phosphorylated at serine 10 during mitosis and found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
Other Names:
H3
Structure:
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution:
Nucleus
Function:
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Regulation:
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Interaction:
Two molecules of H3 form a heterotetramer with two molecules of H4.
Antigen References:
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Hans F, et al. 2001. Oncogene 20:3021.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Histone H3 Phospho (Ser10)
293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).
Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.
Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10)
Hela cells were treated with 20 µM of Nocodazole for 24 hours then fixed, permeabilized with 70% ethanol, and then stained with anti-Histone H3 Phospho-Ser10 (clone 11D8) Alexa Flour® 488 or mouse IgG2b, κ Alexa Flour® 488 isotype control.
