Antibodies
Login
Search   
AKP Streptavidin
AKP Streptavidin
405211 1 ml $110.00     
Product Details
Formulation: HEPES buffer containing 1mM Mg2+, 0.1mM Zn2+, 0.09% sodium azide pH 7.5 and 0.1% BSA added as stabilizer.
Preparation: Streptavidin is conjugated with AKP under optimal conditions. The solution is free of unconjugated AKP and unconjugated Streptavidin.
Storage & Handling: The streptavidin-AKP solution should be stored undiluted at 4°C. Do not freeze.
Application:

ELISA - Quality tested
ELISPOT, IHC, WB - Reported in the literature
Recommended Usage:

Each lot of this Streptavidin-AKP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:200 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

Streptavidin-AKP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-AKP is recommended for ELISA and Western blotting, when used with the relevant substrate system.



Description:

Streptavidin binds to biotin with high affinity. Streptavidin-AKP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, AKP will act on soluble substrates, such as pNPP, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, AKP will act on precipitating substrates, such as NBT/BCIP, to yield a colorimetric reaction.
Other Names: Streptavidin-Alkaline Phosphatase, SAv-AKP
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: AKP Streptavidin, AKP, Streptavidin-Alkaline Phosphatase, SAv-AKP, Mouse, Human, Rat, Immunology, Antibodies
Related Products
DescriptionCloneApplications
ELISA Wash Buffer (20X)ELISA
ELISA Coating Buffer (5X)ELISA
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Copyright © 2012 BioLegend, Inc. All rights reserved.