||Induction of a cellular active state from a normal resting state.
||Use of a cell-surface targeted antibody to activate a biological response. The response can be either positive or negative depending on the function of the targeted protein.
||A process of programmed cell death.
||Use of a recombinant protein to induce a biological response.
||Use of a cell-surface targeted antibody to block a biological response.
||Binding of a cell to a surface, extracellular matrix or another cell using cell adhesion molecules such as selectins, integrins, and cadherins.
||Characterization and determination of the immunophenotype of a cell population.
||Use of an antibody to precipitate a protein-DNA complex in order to determine protein binding sites on DNA.|
|Complement Mediated Cell Depletion
||Use of an antibody to target cells for complement-mediated cell lysis.|
||Enhancement of a primary stimulatory signal by targeting a different molecule.
||Use an antibody to trigger a cytotoxic cellular response that is not apoptotic.
||In vivo, refers to injection of antibody to deplete targeted cell types. In vitro, refers to addition of an antibody in culture to deplete target cells.
||Enzyme-linked Immunosorbent Assay
- LEGEND MAX™ Kits include: Pre-coated plates, detection antibody, recombinant standards, avidin-HRP, and all the required buffers.
- ELISA MAX™ Deluxe sets include: ELISA Plates, Capture and detection antibodies, recombinant standards, avidin-HRP, assay diluents, coating buffer, TMB substrate.
- ELISA MAX™ Standard sets include: Capture and detection antibodies, recombinant standards, and avidin-HRP.
||Enzyme-linked Immunosorbent Spot
||Use of a cell-surface targeted antibody to induce, modify, or block a biological response.
||Flow cytometric analysis of antibody surface-stained cells.
|Intracellular Staining for Flow Cytometry
||Flow cytometric analysis of intracellularly-stained cells.
||Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies.
||Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides.
||Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded.
||Precipitation of a target protein from solution using an antibody.
||Neutralization of a soluble factor by an antibody.
||Single cell analysis of antigen expression using a novel elemental mass-spectrometry detection technology (Inductively Coupled Plasma Mass Spectrometer, ICP-MS) that utilizes isotope-labeled antibodies.
||Process designed to isolate a single type of protein from a complex mixture.
||A very sensitive in vitro assay technique used to measure concentrations of antigens (for example, hormone levels in the blood) by use of antibodies and radioactive materials.
||Induction of a measurable cellular response to external stimuli, such as an antibody.
||Use of gel electrophoresis to separate proteins by size, followed by detection with an antibody.