| Application |
Abbreviation |
Description |
| Activation |
Activ |
Induction of a cellular active state from a normal resting state. |
| Apoptosis |
Apop |
A process of programmed cell death. |
| Bioassay |
BA |
Use of a recombinant protein to induce a biological response. |
| Blocking |
Block |
Use of a cell-surface targeted antibody to block a biological response. |
| Chromatin Immunoprecipitation |
ChIP |
Use of an antibody to precipitate a protein-DNA complex in order to determine protein binding sites on DNA. |
| Complement Mediated Cell Depletion |
C' |
Use of an antibody to target cells for complement-mediated cell lysis. |
| Costimulation |
Costim |
Enhancement of a primary stimulatory signal by targeting a different molecule. |
| Cytotoxicity |
Cyt |
Use an antibody to trigger a cytotoxic cellular response that is not apoptotic. |
| Depletion |
Depletion |
In vivo, refers to injection of antibody to deplete targeted cell types. In vitro, refers to addition of an antibody in culture to deplete target cells.
|
| ELISA |
ELISA |
Enzyme-linked Immunosorbent Assay
- LEGEND MAX™ Kits include: Pre-coated plates, detection antibody, recombinant standards, avidin-HRP, and all the required buffers.
- ELISA MAX™ Deluxe sets include: ELISA Plates, Capture and detection antibodies, recombinant standards, avidin-HRP, assay diluents, coating buffer, TMB substrate.
- ELISA MAX™ Standard sets include: Capture and detection antibodies, recombinant standards, and avidin-HRP. |
| ELISPOT |
ELISPOT |
Enzyme-linked Immunosorbent Spot |
| Functional Assay |
FA |
Use of a cell-surface targeted antibody to induce, modify, or block a biological response. |
| Flow Cytometry |
FC |
Flow cytometric analysis of antibody surface-stained cells. |
| Intracellular Staining for Flow Cytometry |
ICFC |
Flow cytometric analysis of intracellularly-stained cells. |
| Immunocytochemistry |
ICC |
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies. |
| Immunofluorescence Microscopy |
IF |
Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides. |
| Immunohistochemistry |
IHC |
Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded. |
| Immunoprecipitation |
IP |
Precipitation of a target protein from solution using an antibody. |
| Neutralization |
Neut |
Neutralization of a soluble factor by an antibody. |
| Mass Cytometry |
CyTOF® |
Single cell analysis of antigen expression using a novel elemental mass-spectrometry detection technology (Inductively Coupled Plasma Mass Spectrometer, ICP-MS) that utilizes isotope-labeled antibodies. |
| Stimulation |
Stim |
Induction of a measurable cellular response to external stimuli, such as an antibody. |
| Western Blotting |
WB |
Use of gel electrophoresis to separate proteins by size, followed by detection with an antibody. |
