CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. Note, BioLegend does not recommend use of 7-AAD with either PE-Cy5 or PE-Cy7 antibody conjugates.
Application Notes:
7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.
Description:
7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Other Names:
Viability dye, dead cell exclusion dye, cell viability dye
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