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7-AAD Viability Staining Solution
7-AAD Viability Staining Solution
420403 200 tests $25.00     
420404 500 tests $50.00     
Product Details
Formulation: PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation: CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Storage & Handling: Protect from light. Store at 4°C.
Application:

FC - Quality tested
Recommended Usage:

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 
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Application
Notes:

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.
Application
References:

1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed



Description:

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Other Names: Viability dye, dead cell exclusion dye, cell viability dye
Antigen
References:

1. Schmid I, et al. 1992. Cytometry. 13:204
GeneID: NA
Latest Publications: View the latest 7-AAD articles on HighwirePress.com
UniProt: View information about 7-AAD on UniProt.org
Keywords: 7-AAD Viability Staining Solution, Solution, Viability dye, dead cell exclusion dye, cell viability dye, Immunology, Antibodies
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Cell Staining BufferFC, ICC, ICFC
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