Antibodies
Search   

CatalogProductSizeQtyPriceExt. Price 
Total$0.00 
 

Log in to your account to view/modify your save items list
 
 
7-AAD Viability Staining Solution
7-AAD Viability Staining Solution
420403 200 tests $25.00     
420404 500 tests $50.00     
Product Details
 
Formulation: PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation: CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Storage & Handling: Protect from light. Store at 4°C.
Application:

FC - Quality tested
Recommended Usage:

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 
Excitation
Laser: 		Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
Notes:

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.
Application
References:

  Publication Library

See 7-AAD spectral data



Description:

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
  Learn more about Live Cell/Dead Cell Discrimination.
Other Names: Viability dye, dead cell exclusion dye, cell viability dye
Antigen
References:

1. Schmid I, et al. 1992. Cytometry. 13:204
GeneID: NA
Latest Publications: View the latest 7-AAD articles on HighwirePress.com
Keywords: 7-AAD Viability Staining Solution, Solution, Viability dye, dead cell exclusion dye, cell viability dye, Flow Cytometry, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Copyright © 2013 BioLegend, Inc. All rights reserved.