CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Storage & Handling:
Protect from light. Store at 4°C.
Application:
FC
Recommended Usage:
For dead cell exclusion, use 10 µl per test and incubate for 5-10 minutes before analysis.
Application Notes:
7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.
Description:
7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Other Names:
Viability dye, dead cell exclusion dye, cell viability dye
