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PE/Cy7 anti-human CD197 (CCR7) Antibody
PE/Cy7 anti-human CD197 (CCR7) Antibody
353225 25 tests $155.00 Qty:
    
353226 100 tests $325.00 Qty:
    

Product Details

 
Clone: G043H7 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:PE/Cy7 Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human, Cross-Reactivity: Rhesus, Cynomolgus, Baboon
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
PE/Cy7 anti-human CD197 (CCR7) Antibody G043H7 Image 1
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cy7 (top) or mouse IgG2a, κ PE/Cy7 isotype control (bottom).

PE/Cy7 anti-human CD197 (CCR7) Antibody G043H7 Image 2


Compare all formats

See PE/Cy7 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Other Names: BLR2, CDw197, EBI1, CMKBR7
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand Receptor: CCL19 and CCL21.
Antigen
References:

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.

GeneID: 1236
Latest Publications: View the latest CD197 articles on HighwirePress.com
UniProt: View information about CD197 on UniProt.org
Keywords: PE/Cy7 anti-human CD197 (CCR7), G043H7, PE/Cy7, BLR2, CDw197, EBI1, CMKBR7 , Human, Cross-Reactivity: Rhesus, Cynomolgus, Baboon, Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
Does staining at RT or even at 37 degrees help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at RT or at 37 degrees if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD197 (CCR7)G043H7FC
Alexa Fluor® 647 anti-human CD197 (CCR7)G043H7FC
APC anti-human CD197 (CCR7)G043H7FC
APC/Cy7 anti-human CD197 (CCR7)G043H7FC
Biotin anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 421™ anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 510™ anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 605™ anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 650™ anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 711™ anti-human CD197 (CCR7)G043H7FC
Brilliant Violet 785™ anti-human CD197 (CCR7)G043H7FC
FITC anti-human CD197 (CCR7)G043H7FC
LEAF™ Purified anti-human CD197 (CCR7)G043H7FC, Block
Pacific Blue™ anti-human CD197 (CCR7)G043H7FC
PE anti-human CD197 (CCR7)G043H7FC
PE/Dazzle™ 594 anti-human CD197 (CCR7)G043H7FC
PerCP/Cy5.5 anti-human CD197 (CCR7)G043H7FC
Purified anti-human CD197 (CCR7)G043H7FC
Purified anti-human CD197 (CCR7) (MaxPar® Ready)G043H7FC, CyTOF®


Related Pathways

Pathway
Chemokine Receptor Biology
Dendritic Cells
T Follicular Helper Cell Pathway
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > Dendritic cells > CD197 (CCR7)
Immunology > Human Immunology > Th1/Th2 > CD197 (CCR7)
Immunology > Human Immunology > Cytokine and Chemokine Receptors > CD197 (CCR7)
Immunology > Human Immunology > NK/NKT > CD197 (CCR7)
G043H7
Immunology > Human Immunology > CD and Related Molecules > CD197 (CCR7) > G043H7

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2014-04-08
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with purified anti-human CCR7/CD197 (clone G043H7) (filled histogram) or mouse IgG2a isotype control (open histogram), followed by goat anti-mouse IgG-PE.

  • Alexa Fluor® 488 anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CCR7/CD197 (clone G043H7) Alexa Fluor® 488 (top) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (bottom).





  • Brilliant Violet 421™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).





  • PE anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CCR7/CD197 (clone G043H7) PE (top) or mouse IgG2a, κ PE isotype control (bottom).





  • APC/Cy7 anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) APC/Cy7 (top) or mouse IgG2a, κ APC/Cy7 isotype control (bottom).





  • Pacific Blue™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD197 (clone G043H7) Pacific Blue™ (top) or mouse IgG2a, κ Pacific Blue™ isotype control (bottom).





  • APC anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7 (clone G043H7) APC (top) or mouse IgG2a, κ APC isotype control (bottom).





  • FITC anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD197 (clone G043H7) FITC (top) or mouse IgG2a, κ FITC isotype control (bottom).





  • Alexa Fluor® 647 anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) Alexa Fluor® 647 (top) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (bottom).





  • PerCP/Cy5.5 anti-human CD197 (CCR7)
    Human peripheral blood leucocytes were

    Human peripheral blood leucocytes were stained with CD3 FITC and CD197 (clone G043H7) PerCP/Cy5.5 (top) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (bottom).





  • LEAF™ Purified anti-human CD197 (CCR7)
    Chemotaxis of human CCR7-transfected BaF3

    Chemotaxis of human CCR7-transfected BaF3 cells was performed in the presence of 15 ng/ml of recombinant CCL19. Addition of LEAF™ purified anti-human CCR7 (clone G043H7) inhibited migration of hCCR7 transfected BaF3 cells (ED 1.42 µg/ml).

  • Brilliant Violet 605™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet 605™.

  • PE/Cy7 anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cy7 (top) or mouse IgG2a, κ PE/Cy7 isotype control (bottom).





  • Brilliant Violet 711™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (CCR7, clone G043H7) Brilliant Violet 711™.

  • Brilliant Violet 785™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD197 (clone G043H7) Brilliant Violet 510™ (top) or mouse IgG2a, κ Brilliant Violet 510™ isotype control (bottom).





  • Brilliant Violet 650™ anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7 (clone G043H7) Brilliant Violet 650™ (top) or mouse IgG2a, κ Brilliant Violet 650™ isotype control (bottom).





  • PE/Dazzle™ 594 anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD197 (clone G043H7) PE/Dazzle™ 594 (top) or mouse IgG2a, κ PE/Dazzle™ 594 isotype control (bottom).





  • Biotin anti-human CD197 (CCR7)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD197 (clone G043H7) (filled histogram) or biotinylated mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.

  • Purified anti-human CD197 (CCR7) (MaxPar® Ready)
    Human PBMCs stained with 170Er-anti-CD3

    Human PBMCs stained with 170Er-anti-CD3 (UCHT1) and 159Tb-anti-CD197 (G043H7). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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