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PE anti-mouse CLEC9A (DNGR1) Antibody
PE anti-mouse CLEC9A (DNGR1) Antibody
143503 25 µg $95.00 Qty:
    
143504 100 µg $275.00 Qty:
    

Product Details

 
Clone: 7H11 (See other available formats)
Isotype: Rat IgG1, κ
Isotype Control:PE Rat IgG1, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.

Application
References:

  Publication Library
PE anti-mouse CLEC9A (DNGR1) Antibody 7H11 Image 1
C57BL/6 mouse splenocytes were stained with CD11c APC, CD8a PerCP, B220 FITC, and CLEC9a (clone 7H11) PE (top) or rat IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD8α+ and B220-/CD4- cells.

PE anti-mouse CLEC9A (DNGR1) Antibody 7H11 Image 2


Compare all formats

See PE spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CLEC9A, also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Other Names: DNGR-1, C-type lectin domain family 9 member A
Structure: Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution: Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells
Function: Cross present antigen to CTL
Ligand Receptor: Ubiquitous preformed acid-labile protein associated ligand(s)
Interaction: Necrotic cells
Antigen
References:

Poulin LF, et al. 2012. Blood 119:6052.

GeneID: 232414
Latest Publications: View the latest CLEC9A articles on HighwirePress.com
UniProt: View information about CLEC9A on UniProt.org
Keywords: PE anti-mouse CLEC9A (DNGR1), 7H11, PE, DNGR-1, C-type lectin domain family 9 member A , Mouse, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Purified anti-mouse CLEC9A (DNGR1)7H11FC, ELISA, IF, IP, WB


Related Pathways

Pathway
Dendritic Cells


Related Categories

Category
Immunology > Mouse Immunology > CD and Related Molecules > CLEC9A > 7H11
Immunology > Mouse Immunology > Innate Immunity > CLEC9A
Immunology > Mouse Immunology > MHC Antigens > CLEC9A
Immunology > Mouse Immunology > Dendritic cells > CLEC9A (DNGR1)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-03-15
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse CLEC9A (DNGR1)
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD11c APC, CD8a PerCP, B220 FITC, and CLEC9a (clone 7H11) PE (top) or rat IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD8α+ and B220-/CD4- cells.





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