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PE anti-mouse CD370 (CLEC9A, DNGR1) Antibody
Cat. # Size Price  

143503 25 µg $95.00
143504 100 µg $275.00
Product Details

Clone: 7H11 (See other available formats)
Isotype: Rat IgG1, κ
Isotype Control:PE Rat IgG1, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.

(PubMed link indicates BioLegend citation)
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RRID: AB_11147366 (BioLegend Cat. No. 143503)
AB_11147953 (BioLegend Cat. No. 143504)
 Image 1
C57BL/6 mouse splenocytes were stained with CD11c APC, CD8a PerCP, B220 FITC, and CD370 (CLEC9A, clone 7H11) PE (top) or rat IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD8α+ and B220-/CD4- cells.

 Image 2

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See PE spectral data


Antigen Details

Description: CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
Other Names: DNGR-1, C-type lectin domain family 9 member A, CD370
Structure: Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution: Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells
Function: Cross present antigen to CTL
Ligand Receptor: Ubiquitous preformed acid-labile protein associated ligand(s)
Interaction: Necrotic cells
Poulin LF, et al. 2012. Blood 119:6052.
GeneID: 232414
UniProt: View information about CD370 on
Keywords: PE anti-mouse CD370 (CLEC9A, DNGR1), 7H11, PE, DNGR-1, C-type lectin domain family 9 member A, CD370, Mouse, Flow Cytometry, Immunology, Antibodies
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Version: 3 Revision Date: 2015-01-15
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse CD370 (CLEC9A, DNGR1)
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD11c APC, CD8a PerCP, B220 FITC, and CD370 (CLEC9A, clone 7H11) PE (top) or rat IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD8α+ and B220-/CD4- cells.

  • APC anti-mouse CD370 (CLEC9A, DNGR1)
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD11c PE, CD8α PerCP, CD45R/B220 FITC, CD4 FITC, and CD370 (CLEC9A, clone 7H11) APC (top) or rat IgG1, κ APC isotype control (bottom). Data shown was gated on CD8α+, CD45R/ B220-, and CD4- cells.

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