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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
C57BL/6 mouse splenocytes were stained with CD8 PerCP, CD11c APC, and CLEC12A (clone 5D3/CLEC12A) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was gated on CD8 positive cell population.
CLEC12A, also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family. Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases. Mouse CLEC12A is expressed on CD8+ dendritic cells (DCs), a proportion of CD8- dendritc cells, plasmacytoid DCs, monoctyes, macrophages, granulocytes, and B cells.
Other Names:
CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A
Structure:
Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution:
CD8+ dendritic cells (DCs), a proportion of CD8- dendritic cells, plasmacytoid DCs, monocytes, macrophages, granulocytes, B cells
Function:
Negative regulator of granuocyte and monocyte function
Interaction:
PTPN6 and PTPN11
Antigen References:
1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. 2. Chen CH, et al. 2006. Blood 107:1459. 3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CLEC12A
C57BL/6 mouse splenocytes were stained with CD8 PerCP, CD11c APC, and CLEC12A (clone 5D3/CLEC12A) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was gated on CD8 positive cell population.
PE anti-mouse CLEC12A
C57BL/6 mouse splenocytes were stained with CD8 PerCP, CD11c APC, and CLEC12A (clone 5D3/CLEC12A) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was gated on CD8 positive cell population.
