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Brilliant Violet 650™ anti-human CD127 (IL-7Rα) Antibody
Brilliant Violet 650™ anti-human CD127 (IL-7Rα) Antibody
351325 25 tests $205.00 Qty:
    
351326 100 tests $405.00 Qty:
    

Product Details

 
Clone: A019D5 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: Recombinant human CD127
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
Excitation
Laser:
Violet Laser (405 nm)
COA:
Enter Lot#:   
Brilliant Violet 650™ anti-human CD127 (IL-7Rα) Antibody A019D5 Image 1
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 650™.


Compare all formats

See Brilliant Violet 650™ spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Other Names: IL-7 receptor α chain, IL-7Rα
Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand Receptor: IL-7
Antigen
References:

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

GeneID: 3575
Latest Publications: View the latest CD127 articles on HighwirePress.com
UniProt: View information about CD127 on UniProt.org
Keywords: Brilliant Violet 650™ anti-human CD127 (IL-7Rα), A019D5, Brilliant Violet 650™, BV650, IL-7 receptor α chain, IL-7Rα, Human, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)A019D5FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)A019D5FC
APC anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)A019D5FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)A019D5FC
FITC anti-human CD127 (IL-7Rα)A019D5FC
Pacific Blue™ anti-human CD127 (IL-7Rα)A019D5FC
PE anti-human CD127 (IL-7Rα)A019D5FC
PE/Cy5 anti-human CD127 (IL-7Rα)A019D5FC
PE/Cy7 anti-human CD127 (IL-7Rα)A019D5FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)A019D5FC
PerCP/Cy5.5 anti-human CD127 (IL-7Rα)A019D5FC
Purified anti-human CD127 (IL-7Rα)A019D5FC


Related Pathways

Pathway
Cancer Immunoediting
Innate Immunity
T Follicular Helper Cell Pathway
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > CD and Related Molecules > CD127 (IL-7Rα) > A019D5
Immunology > Human Immunology > Cytokine and Chemokine Receptors > CD127 (IL-7Rα)
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD127 (IL-7Rα)
Immunology > Human Immunology > B Cells > CD127 (IL-7Rα)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with purified CD127 (clone A019D5) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by anti-mouse IgG FITC.

  • PE anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) PE (top) or mouse IgG1 PE isotype control (bottom).





  • Pacific Blue™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 PE and CD127 (clone A019D5) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).





  • Brilliant Violet 421™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • FITC anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) FITC (top) or mouse IgG1 FITC isotype control (bottom).





  • Alexa Fluor® 488 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) Alexa Fluor® 488 (top) or mouse IgG1 Alexa Fluor® 488 isotype control (bottom).





  • APC anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).





  • Alexa Fluor® 647 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom).





  • PE/Cy7 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cy7 (top) or mouse IgG1 PE/Cy7 isotype control (bottom).





  • PerCP/Cy5.5 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PerCP/Cy5.5 (top) or mouse IgG1 PerCP/Cy5.5 isotype control (bottom).





  • Brilliant Violet 570™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • PE/Cy5 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cy5 (top) or mouse IgG1, κ PE/Cy5 isotype control (bottom).





  • Brilliant Violet 650™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 650™.

  • Brilliant Violet 711™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD127 (clone A019D5) Brilliant Violet 711™.

  • Brilliant Violet 785™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD127 (clone A019D5) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD127 (clone A019D5) Brilliant Violet 510™ (filled histogram) or mouse IgG1 Brilliant Violet 510™ isotype control (open histogram).

  • Brilliant Violet 605™ anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).





  • PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/ Dazzle™ 594 isotype control (bottom).





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