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Brilliant Violet 650™ anti-human CD19 Antibody
Brilliant Violet 650™ anti-human CD19 Antibody
302237 25 tests $195.00 Qty:
    
302238 100 tests $385.00 Qty:
    

Product Details

 
Clone: HIB19 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
V CD19.11
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
Excitation
Laser:
Violet Laser (405 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302214).

Application
References:

  Publication Library
Brilliant Violet 650™ anti-human CD19 Antibody HIB19 Image 1
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 650™.


Compare all formats

See Brilliant Violet 650™ spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Other Names: B4
Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: B lineage (except plasma cells), follicular dendritic cells
Function: B cell activation and differentiation
Ligand Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Antigen
References:

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

GeneID: 930
Latest Publications: View the latest CD19 articles on HighwirePress.com
UniProt: View information about CD19 on UniProt.org
Keywords: Brilliant Violet 650™ anti-human CD19, HIB19, Brilliant Violet 650™, BV650, B4, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunology, Antibodies


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DescriptionCloneApplications
Brilliant Violet 650™ Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC
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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD19HIB19FC, IF
Alexa Fluor® 594 anti-human CD19HIB19IF
Alexa Fluor® 647 anti-human CD19HIB19FC, IF
Alexa Fluor® 700 anti-human CD19HIB19FC
APC anti-human CD19HIB19FC
APC/Cy7 anti-human CD19HIB19FC
Biotin anti-human CD19HIB19FC
Brilliant Violet 421™ anti-human CD19HIB19FC, IF
Brilliant Violet 510™ anti-human CD19HIB19FC
Brilliant Violet 570™ anti-human CD19HIB19FC
Brilliant Violet 605™ anti-human CD19HIB19FC
Brilliant Violet 711™ anti-human CD19HIB19FC
Brilliant Violet 785™ anti-human CD19HIB19FC
FITC anti-human CD19HIB19FC
LEAF™ Purified anti-human CD19HIB19FC, Block, IHC, CyTOF®
Pacific Blue™ anti-human CD19HIB19FC
PE anti-human CD19HIB19FC
PE/Cy5 anti-human CD19HIB19FC
PE/Cy7 anti-human CD19HIB19FC
PE/Dazzle™ 594 anti-human CD19HIB19FC
PerCP anti-human CD19HIB19FC
PerCP/Cy5.5 anti-human CD19HIB19FC
Purified anti-human CD19HIB19FC, IHC, CyTOF®
Purified anti-human CD19 (MaxPar® Ready)HIB19FC, CyTOF®


Related Pathways

Pathway
B cell Development and Activation
B-Cell Receptor Pathway
Innate Immunity


Related Categories

Category
Immunology > Non-Human Primate Immunology > CD19
Immunology > Human Immunology > Dendritic cells > CD19
Immunology > Human Immunology > Stem Cells > CD19 (B4)
Immunology > Human Immunology > CD and Related Molecules > CD19 (B4) > HIB19
Immunology > Human Immunology > B Cells > CD19 (B4) > HIB19
Immunology > Human Immunology > Costimulatory Molecules > CD19 (B4) > HIB19

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-12-18
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 APC

  • Biotin anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HIB19, followed by Sav-PE

  • FITC anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 FITC

  • LEAF™ Purified anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HIB19, followed by anti-mouse IgGs FITC

  • PE anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 PE

  • PE/Cy5 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 PE/Cy5

  • Purified anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC

  • APC/Cy7 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 APC/Cy7

  • PE/Cy7 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 PE/Cy7

  • Alexa Fluor® 488 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 Alexa Flour® 488

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (yellow), 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green), and 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 594 (red) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

  • Alexa Fluor® 647 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 Alexa Flour® 647

    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA) and then stained with 5 µg/ml CD4 (clone OKT4) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone HIB19) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD56 (clone HCD56) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone HIB19) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Pacific Blue™ anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™

  • Alexa Fluor® 700 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 Alexa Fluor® 700

  • PerCP anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 PerCP

  • PerCP/Cy5.5 anti-human CD19
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIB19 PerCP/Cy5.5

  • Brilliant Violet 421™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD19 (clone HIB19) Brilliant Violet 421™ (blue) and 5µg/ml CD3 (clone UCHT1) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Brilliant Violet 570™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • Brilliant Violet 650™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 650™.

  • Brilliant Violet 785™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 APC and CD19 (clone HIB19) Brilliant Violet 510™.

  • Brilliant Violet 605™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).





  • Brilliant Violet 711™ anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 711™ (top) or mouse IgG1, κ Brilliant Violet 711™ isotype control (bottom).





  • Purified anti-human CD19 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 142Nd-anti-CD19 (HIB19). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-human CD19
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 594 (red), 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (yellow), and 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

  • PE/Dazzle™ 594 anti-human CD19
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

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