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Brilliant Violet 605™ anti-mouse CD25 Antibody
Cat. # Size Price  

102035 125 µl $205.00
    
Reviews (1)
102036 50 µg $245.00
    
Reviews (1)
Product Details

Clone: PC61 (See other available formats)
Isotype: Rat IgG1, λ
Reactivity: Mouse
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 605™ and unconjugated antibody.
Concentration: µg size: 0.2 mg/ml
µl size: lot-specific
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.3 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
Excitation
Laser:
Violet Laser (405 nm)
Application
Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102014). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
  Publication Library
 Image 1
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 (clone PC61) Brilliant Violet 605™.


Compare all formats

See Brilliant Violet 605™ spectral data





 


Antigen Details

Description: CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Other Names: IL-2Rα, Ly-43, p55, Tac
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor
Ligand Receptor: IL-2
Antigen
References:
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
GeneID: 16184
UniProt: View information about CD25 on UniProt.org
Keywords: Brilliant Violet 605™ anti-mouse CD25, PC61, Brilliant Violet 605™, BV605, IL-2Rα, Ly-43, p55, Tac, Mouse, Flow Cytometry, Immunology, Antibodies
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Cell Surface Immunofluorescence Staining Protocol
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Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD25
    Con A-stimulated (3 days)splenocytes stained

    Con A-stimulated (3 days)splenocytes stained with PC61 APC

  • Biotin anti-mouse CD25
    Con A-stimulated C57BL/6 splenocytes (3

    Con A-stimulated C57BL/6 splenocytes (3 days) stained with PC 61 biotin, followed by Sav-PE

  • FITC anti-mouse CD25
    Con-A Stimulated (3 days) BALB/c

    Con-A Stimulated (3 days) BALB/c mouse splenocytes stained with PC61 FITC

  • LEAF™ Purified anti-mouse CD25
    Con A-stimulated (3 days) BALB/c

    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with LEAF™ purified PC61, followed by anti-rat IgG FITC

  • PE anti-mouse CD25
    Con A-stimulated (3 days) BALB/c

    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 PE

  • PE/Cy5 anti-mouse CD25
    Con A-stimulated (day-3) C57BL/6 mouse

    Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 PE/Cy5

  • Purified anti-mouse CD25
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC

  • PE/Cy7 anti-mouse CD25
    Con A-stimulated (2 days) BALB/c

    Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cy7

  • Alexa Fluor® 488 anti-mouse CD25
    Con A-stimulated (3 days) BALB/c

    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-mouse CD25
    Con A-stimulated (3 days) BALB/c

    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 Alexa Fluor® 647

  • Pacific Blue™ anti-mouse CD25
    Con A-stimulated (3 days) C57BL/6

    Con A-stimulated (3 days) C57BL/6 mouse splenocytes stained with PC61 Pacific Blue™

  • Alexa Fluor® 700 anti-mouse CD25
    Con A-stimulated (day-3) C57BL/6 mouse

    Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with Alexa Fluor® 700

  • APC/Cy7 anti-mouse CD25
    Con A-stimulated (day-3) C57BL/6 mouse

    Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cy7

  • PerCP/Cy5.5 anti-mouse CD25
    Con A-stimulated C57BL/7 splenocytes (Day

    Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC61 PerCP/Cy5.5

  • PerCP anti-mouse CD25
    Con A-stimulated C57BL/7 splenocytes (Day

    Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC61 PerCP

  • Brilliant Violet 421™ anti-mouse CD25
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD4 FITC and CD25 (clone PC61) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • Brilliant Violet 605™ anti-mouse CD25
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 (clone PC61) Brilliant Violet 605™.

  • Brilliant Violet 650™ anti-mouse CD25
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD4 FITC and CD25 (clone PC61) Brilliant Violet 650™.

  • Ultra-LEAF™ Purified anti-mouse CD25
    Con A-stimulated (3 days) BALB/c

    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with LEAF™ purified PC61, followed by anti-rat IgG FITC

  • Brilliant Violet 510™ anti-mouse CD25
    Con A-stimulated (3 days) Balb/c

    Con A-stimulated (3 days) Balb/c splenocytes were stained with CD25 (clone PC61) Brilliant Violet 421™ (filled histogram). Unstained control cells are represented by the open histogram.

  • PE/Dazzle™ 594 anti-mouse CD25
    Con A-stimulated (3 days) C57BL/6

    Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 (clone PC61) PE/Dazzle™ 594 (filled histogram). Unstained control cells are represented by the open histogram.

  • Brilliant Violet 711™ anti-mouse CD25
    Con A-stimulated (3 days) C57BL/6

    Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 (clone PC61) Brilliant Violet 711™ (filled histogram). Unstained control cells are represented by the open histogram.

  • Brilliant Violet 785™ anti-mouse CD25
    Con A-stimulated (3 days) C57BL/6

    Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 (clone PC61) Brilliant Violet 785™ (filled histogram). Unstained control cells are represented by the open histogram.

  • Alexa Fluor® 594 anti-mouse CD25
    C57BL/6 mice were intraperitoneally injected

    C57BL/6 mice were intraperitoneally injected with 100 µg LEAF™ purified DR3 (TNFRSF25) for five days. Then, frozen spleen sections were fixed with acetone for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 30 minutes at room temperature. Then the sections were stained with 2.5 µg/ml of CD25 (clone PC61) Alexa Fluor® 594 (red) and anti-mouse/human CD45R/B220 Brilliant Violet 421™ (blue) overnight at 4°C. The image was captured with a 10X objective.

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