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PE anti-human CD89 Antibody
PE anti-human CD89 Antibody
354103 25 tests $105.00 Qty:
    
354104 100 tests $245.00 Qty:
    

Product Details

 
Clone: A59 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Workshop
Number:
V MR30
Reactivity: Human, Cross-Reactivity4: Cynomolgus, Rhesus
Immunogen: Ag8.653 myeloma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
References:

  Publication Library
PE anti-human CD89 Antibody A59 Image 1
Human peripheral blood granulocytes were stained with anti-human CD89 PE (clone A59) (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).


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See PE spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD89, also known as FcαR, is a 55-100 kD glycosylated protein.  It belongs to the immunoglobulin gene family.  It is expressed on granulocytes, monocytes, and macrophages but is absent on T cells.  It can interact with IgA aggregates and plays an important role in IgA mediated immune responses.

Other Names: FcαR
Structure: Member of the immunoglobulin gene family and is a transmembrane glycoprotein, 55-100 kD
Distribution: Granulocytes, neutrophils, eosinophils, monocytes, macrophages
Function: Interacts with IgA opsonized targets and induces immunological processes such as phagocytosis, ADCC, and release of inflammatory mediators
Ligand Receptor: IgA (monomeric, dimeric, polymeric and secretory IgA)
Antigen
References:

1. Patry C, et al. 1996. J. Immunol. 156:4442.
2. de Wit, et al. 1995. J. Immunol. 155:1203.
3. Honorio-França AC, et al. 2001. J. Leukoc. Biol. 69:289.

GeneID: 2204
Latest Publications: View the latest CD89 articles on HighwirePress.com
UniProt: View information about CD89 on UniProt.org
Keywords: PE anti-human CD89, A59, PE, FcαR, Human, Cross-Reactivity4: Cynomolgus, Rhesus, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
BioLegend 354104 (FC) /4 2012-08-21
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Other Formats

DescriptionCloneApplications
Purified anti-human CD89A59FC


Related Pathways

Pathway


Related Categories

Category
Immunology > Human Immunology > CD and Related Molecules > CD89 > A59
Immunology > Human Immunology > Monocyte/Macrophage > CD89
CD89

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-10-08
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD89
    Human peripheral blood granulocytes were

    Human peripheral blood granulocytes were stained with anti-human CD89 PE (clone A59) (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

  • Purified anti-human CD89
    Human peripheral blood granulocytes were

    Human peripheral blood granulocytes were stained with purified anti-human CD89 (clone A59, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.

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