The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC2-4,7,8
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.1 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
ELISA3,4 and ELISPOT5 Capture: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 564101) as the standard. Flow Cytometry2-4,7,8: The fluorochrome-labeled TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization6: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906). Additional reported applications (for the relevant formats) include: Western blotting.
Application References:
1. Kennedy, J., et al., 1996. J. Interferon Cytokine Res. 16: 611. 2. Schubert, D., et al., 2004. J. Immunol. 172: 4503. 3. Infante-Duarte, C., et al., 2000. J. Immunol. 165: 6107. 4. Harrington, L. E., et al., 2005. Nature Immunol. doi:10.1038/ni1254. 5. Nekrasova, T., et al., 2005. J. Immunol. 175: 2734. 6. Yen, D., et al., 2006. J. Clin. Invest. 116: 1310. 7. Ehirchiou, D., et al., 2007. J. Exp. Med. 204:1519. 8. Kang, S. G., et al., 2007. J. Immunol. 179:3724. 9. Hamada, H., et al. 2009. J. Immunol. 182:3469.
PMA/ionomycin-stimulated EL-4 cells intracellularly stained with TC11-8H10.1 Pacific Blue™
Description:
IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-18H10.1 antibody can neutralize IL-17 activity.
Other Names:
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Structure:
Cytokine; dimer; 15 kD (Mammalian)
Ligand Receptor:
IL-17R (CD217)
Cellular Sources:
CD4+ memory T cells
Cellular Targets:
Fibroblasts, epithelial and endothelial cells, stromal cells
Bioactivity/Activities:
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Numasaki, M., et al., 2002. Blood 101:2620. 3. Fossiez, F., et al., 1996. J. Exp. Med. 183:2593. 4. Yao, Z., et al., 1997. Cytokine 9:794. 5. Dong, C. 2006 Nat. Rev. Immunol. 6(4): 329. 6. Hofstetter, HH., et al., 2005 Cell. Immunol. 237(2): 123.
